Relationship between epilarynx tube shape and the radiated sound pressure level during phonation is gender specific.

OBJECTIVE/HYPOTHESIS: The aim of the study was to measure the morphology of the epilaryngeal tube during sustained phonation as a function of loudness variation and to compare subjects of different genders. STUDY DESIGN: This is a prospective study. METHODS: Five female and five male classically trained singers were recorded by magnetic resonance imaging with simultaneous audio recordings while sustaining phonation at three different loudness conditions. Three-dimensional subsections of the vocal tract were segmented on multi-image-based cross-sections. Different volume and area measures were determined and their relation to sound pressure level and loudness condition was analyzed. RESULTS: Male singers tended to narrow the epilaryngeal tube when increasing sound pressure level whereas female singers did not. CONCLUSION: Strategies of vocal tract adjustments during loudness variation in classical singing appear to be gender specific.

Pre-adolescent children exhibit lower aerosol particle volume emissions than adults for breathing, speaking, singing and shouting.

Speaking and singing are activities linked to increased aerosol particle emissions from the respiratory system, dependent on the utilized vocal intensity. As a result, these activities have experienced considerable restrictions in enclosed spaces since the onset of the COVID-19 pandemic due to the risk of infection from the SARS-CoV-2 virus, transmitted by virus-carrying aerosols. These constraints have affected public education and extracurricular activities for children as well, from in-person music instruction to children's choirs. However, existing risk assessments for children have been based on emission measurements of adults. To address this, we measured the particle emission rates of 15 pre-adolescent children, all eight to ten years old, with a laser particle counter for the test conditions: breathing at rest, speaking, singing and shouting. Compared with values taken from 15 adults, emission rates for breathing, speaking and singing were significantly lower for children. Particle emission rates were reduced by a factor of 4.3 across all conditions, whereas emitted particle volume rates were reduced by a factor of 4.8. These data can supplement SARS-CoV-2 risk management scenarios for various school and extracurricular settings.

Voice efficiency for different voice qualities combining experimentally derived sound signals and numerical modeling of the vocal tract.

Purpose: Concerning voice efficiency considerations of different singing styles, from western classical singing to contemporary commercial music, only limited data is available to date. This single-subject study attempts to quantify the acoustic sound intensity within the human glottis depending on different vocal tract configurations and vocal fold vibration. Methods: Combining Finite-Element-Models derived from 3D-MRI data, audio recordings, and electroglottography (EGG) we analyzed vocal tract transfer functions, particle velocity and acoustic pressure at the glottis, and EGG-related quantities to evaluate voice efficiency at the glottal level and resonance characteristics of different voice qualities according to Estill Voice Training®. Results: Voice qualities Opera and Belting represent highly efficient strategies but apply different vowel strategies and should thus be capable of predominate orchestral sounds. Twang and Belting use similar vowels, but the twang vocal tract configuration enabled the occurrence of anti-resonances and was associated with reduced vocal fold contact but still partially comparable energy transfer from the glottis to the vocal tract. Speech was associated with highly efficient glottal to vocal tract energy transfer, but with the absence of psychoactive strategies makes it more susceptible to noise interference. Falsetto and Sobbing apply less efficiently. Falsetto mainly due to its voice source characteristics, Sobbing due to energy loss in the vocal tract. Thus technical amplification might be appropriate here. Conclusion: Differences exist between voice qualities regarding the sound intensity, caused by different vocal tract morphologies and oscillation characteristics of the vocal folds. The combination of numerical analysis of geometries inside the human body and experimentally determined data outside sheds light on acoustical quantities at the glottal level.

Aerosol emission in professional singing of classical music.

In this study, emission rates of aerosols emitted by professional singers were measured with a laser particle counter under cleanroom conditions. The emission rates during singing varied between 753 and 6093 particles/sec with a median of 1537 particles/sec. Emission rates for singing were compared with data for breathing and speaking. Significantly higher emission rates were found for singing. The emission enhancements between singing and speaking were between 4.0 and 99.5 with a median of 17.4, largely due to higher sound pressure levels when singing. Further, significant effects of vocal loudness were found, whereas there were no significant differences between the investigated voice classifications. The present study supports the efforts to improve the risk management in cases of possible aerogenic virus transmission, especially for choir singing.

Aerosol emission of adolescents voices during speaking, singing and shouting.

Since the outbreak of the COVID-19 pandemic, singing activities for children and young people have been strictly regulated with far-reaching consequences for music education in schools and ensemble and choir singing in some places. This is also due to the fact, that there has been no reliable data available on aerosol emissions from adolescents speaking, singing, and shouting. By utilizing a laser particle counter in cleanroom conditions we show, that adolescents emit fewer aerosol particles during singing than what has been known so far for adults. In our data, the emission rates ranged from 16 P/s to 267 P/s for speaking, 141 P/s to 1240 P/s for singing, and 683 P/s to 4332 P/s for shouting. The data advocate an adaptation of existing risk management strategies and rules of conduct for groups of singing adolescents, like gatherings in an educational context, e.g. singing lessons or choir rehearsals.

Printable 3D vocal tract shapes from MRI data and their acoustic and aerodynamic properties.

A detailed understanding of how the acoustic patterns of speech sounds are generated by the complex 3D shapes of the vocal tract is a major goal in speech research. The Dresden Vocal Tract Dataset (DVTD) presented here contains geometric and (aero)acoustic data of the vocal tract of 22 German speech sounds (16 vowels, 5 fricatives, 1 lateral), each from one male and one female speaker. The data include the 3D Magnetic Resonance Imaging data of the vocal tracts, the corresponding 3D-printable and finite-element models, and their simulated and measured acoustic and aerodynamic properties. The dataset was evaluated in terms of the plausibility and the similarity of the resonance frequencies determined by the acoustic simulations and measurements, and in terms of the human identification rate of the vowels and fricatives synthesized by the artificially excited 3D-printed vocal tract models. According to both the acoustic and perceptual metrics, most models are accurate representations of the intended speech sounds and can be readily used for research and education.

How to precisely measure the volume velocity transfer function of physical vocal tract models by external excitation.

Recently, 3D printing has been increasingly used to create physical models of the vocal tract with geometries obtained from magnetic resonance imaging. These printed models allow measuring the vocal tract transfer function, which is not reliably possible in vivo for the vocal tract of living humans. The transfer functions enable the detailed examination of the acoustic effects of specific articulatory strategies in speaking and singing, and the validation of acoustic plane-wave models for realistic vocal tract geometries in articulatory speech synthesis. To measure the acoustic transfer function of 3D-printed models, two techniques have been described: (1) excitation of the models with a broadband sound source at the glottis and measurement of the sound pressure radiated from the lips, and (2) excitation of the models with an external source in front of the lips and measurement of the sound pressure inside the models at the glottal end. The former method is more frequently used and more intuitive due to its similarity to speech production. However, the latter method avoids the intricate problem of constructing a suitable broadband glottal source and is therefore more effective. It has been shown to yield a transfer function similar, but not exactly equal to the volume velocity transfer function between the glottis and the lips, which is usually used to characterize vocal tract acoustics. Here, we revisit this method and show both, theoretically and experimentally, how it can be extended to yield the precise volume velocity transfer function of the vocal tract.

Three-dimensional Vocal Tract Morphology Based on Multiple Magnetic Resonance Images Is Highly Reproducible During Sustained Phonation.

OBJECTIVES: The extraction of a three-dimensional (3D) morphology of the human vocal tract (VT) from magnetic resonance imaging (MRI) during sustained phonation can be used for various analyses like numerical simulations or creating physical models. The precision of visualizing techniques nowadays allows for very targeted acoustical simulation evaluating the influence of subsections of the VT for the transfer function. The aim of the study was to assess the accuracy of the 3D geometry based on MRI data in repetitive trials. STUDY DESIGN: This is a prospective study. METHODS: Four experienced singers underwent an MRI while repeating a specific vocal task 20 times consecutively. Audio recordings were made by means of an optical microphone. Images were restacked and subsections of the VT were segmented on multi-image-based cross sections using a semiautomatic algorithm. Different volume and area measures were evaluated. RESULTS: A high reproducibility of the morphologic data based on multiple images by means of the applied segmentation method could be shown with an overall variation of around 8%. CONCLUSIONS: 3D modeling of the VT during sustained phonation involves a complex experimental setting and elaborate image processing techniques. Functional comparative analysis or acoustical simulations based on such data should take the found variability into account.

Lower Vocal Tract Morphologic Adjustments Are Relevant for Voice Timbre in Singing.

The vocal tract shape is crucial to voice production. Its lower part seems particularly relevant for voice timbre. This study analyzes the detailed morphology of parts of the epilaryngeal tube and the hypopharynx for the sustained German vowels /a/, /e/, /i/, /o/, and /u/ by thirteen male singer subjects who were at the beginning of their academic singing studies. Analysis was based on two different phonatory conditions: a natural, speech-like phonation and a singing phonation, like in classical singing. 3D models of the vocal tract were derived from magnetic resonance imaging and compared with long-term average spectrum analysis of audio recordings from the same subjects. Comparison of singing to the speech-like phonation, which served as reference, showed significant adjustments of the lower vocal tract: an average lowering of the larynx by 8 mm and an increase of the hypopharyngeal cross-sectional area (+ 21:9%) and volume (+ 16:8%). Changes in the analyzed epilaryngeal portion of the vocal tract were not significant. Consequently, lower larynx-to-hypopharynx area and volume ratios were found in singing compared to the speech-like phonation. All evaluated measures of the lower vocal tract varied significantly with vowel quality. Acoustically, an increase of high frequency energy in singing correlated with a wider hypopharyngeal area. The findings offer an explanation how classical male singers might succeed in producing a voice timbre with increased high frequency energy, creating a singer`s formant cluster.

Formant frequencies and bandwidths of the vocal tract transfer function are affected by the mechanical impedance of the vocal tract wall.

The acoustical properties of the vocal tract, the air-filled cavity between the vocal folds and the mouth opening, are determined by its individual geometry, the physical properties of the air and of its boundaries. In this article, we address the necessity of complex impedance boundary conditions at the mouth opening and at the border of the acoustical domain inside the human vocal tract. Using finite element models based on MRI data for spoken and sung vowels /a/, /i/ and /Ω(-1)/ and comparison of the transfer characteristics by analysis of acoustical data using an inverse filtering method, the global wall impedance showed a frequency-dependent behaviour and depends on the produced vowel and therefore on the individual vocal tract geometry. The values of the normalised inertial component (represented by the imaginary part of the impedance) ranged from 250 g/m(2) at frequencies higher than about 3 kHz up to about 2.5 × 10(5) g/m(2)in the mid-frequency range around 1.5-3 kHz. In contrast, the normalised dissipation (represented by the real part of the impedance) ranged from 65 to 4.5 × 10(5) Ns/m(3). These results indicate that structures enclosing the vocal tract (e.g. oral and pharyngeal mucosa and muscle tissues), especially their mechanical properties, influence the transfer of the acoustical energy and the position and bandwidth of the formant frequencies. It implies that the timbre characteristics of vowel sounds are likely to be tuned by specific control of relaxation and strain of the surrounding structures of the vocal tract.

Tonotopic morphometry of the lamina reticularis of the guinea pig cochlea with associated microstructures and related mechanical implications.

Morphometry of the lamina reticularis of the guinea pig cochlea was performed using scanning electron microscopy. Seventy-four geometrical parameters of the lamina reticularis, the bundles of stereocilia, and individual stereocilia, in all rows of hair cells and within the individual hair cells, were measured at ten equally spaced locations along the longitudinal direction of the cochlea. Variations of the parameters versus the longitudinal coordinate were statistically analyzed and fitted with polynomials (constant, linear, or quadratic). Our data show that a unique set of geometrical parameters of inner and outer hair cells is typical for every frequency-dependent position at the lamina reticularis. Morphology of the outer hair cell structures varies more than respective parameters of the inner hair cells. Mechanical modeling using the obtained geometrical parameters provides a novel glance at the mechanical characteristics with respect to the cochlear tonotopy.

Compliance profiles derived from a three-dimensional finite-element model of the basilar membrane.

A finite-element analysis is used to explore the impact of elastic material properties, boundary conditions, and geometry, including coiling, on the spatial characteristics of the compliance of the unloaded basilar membrane (BM). It is assumed that the arcuate zone is isotropic and the pectinate zone orthotropic, and that the radial component of the effective Young's modulus in the pectinate zone decreases exponentially with distance from base to apex. The results concur with tonotopic characteristics of compliance and neural data. Moreover, whereas the maximum compliance in a radial profile is located close to the boundary between the two zones in the basal region, it shifts to the midpoint of the pectinate zone for the apical BM; the width of the profile also expands. This shift begins near the 1 kHz characteristic place for guinea pig and the 2.4 kHz place for gerbil. Shift and expansion are not observed for linear rather than exponential decrease of the radial component of Young's modulus. This spatial change of the compliance profile leads to the prediction that mechanical excitation in the apical region of the organ of Corti is different to that in the basal region.